{"id":1797,"date":"2020-11-24T14:31:07","date_gmt":"2020-11-24T11:31:07","guid":{"rendered":"https:\/\/f-genetics.com\/?post_type=test&p=1797"},"modified":"2026-02-27T15:31:05","modified_gmt":"2026-02-27T12:31:05","slug":"senger","status":"publish","type":"test","link":"https:\/\/f-genetics.com\/en\/senger\/","title":{"rendered":"Sanger sequencing"},"content":{"rendered":"

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Overview<\/div>
Indications<\/div>
Features<\/div>
NGS\u00a0vs Sanger<\/div><\/div><\/div>
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Sequencing<\/h2>\n\n\n\n

The DNA molecule is the carrier of genetic information in almost all living organisms. It is made up of two complementary, i.e. corresponding strands of nucleotides that form a unique sequence.<\/p>\n\n\n\n

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A pair of complementary nucleotides within a DNA sequence is also called a base pair, abbreviated as bp.<\/p>\n\n\n\n

The human genome contains about 3 billion base pairs.<\/p>\n<\/div>\n<\/div>\n\n\n\n

Sequencing is a method used in laboratory practice to determine the sequence of DNA.<\/p>\n\n\n\n

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In diagnostics, it is often used to look for changes in the structure of genes or their regulatory elements that cause disease (pathogenic mutations, variants).<\/p>\n<\/div>\n<\/div>\n\n\n\n

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Sanger sequencing<\/h2>\n\n\n\n
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Up to 98% accuracy<\/span>sequences of up to 1000 nucleotide pairs can be 'read' in a single cycle with a high accuracy of up to 98%<\/span><\/p>\n<\/div>\n\n\n\n

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Sanger sequencing is one of the classic sequencing methods. The method was developed in 1977 by Frederick Sanger and his colleagues. It was used by the Human Genome Project in 1990-2003 to fully decode the human genome for the first time.<\/p>\n\n\n\n


Sanger sequencing is still relevant today and is a routine method both in genetic diagnosis and in research practice. It can be used to 'read' sequences of up to 1000 nucleotide pairs in a single cycle with a high accuracy of up to 98%. <\/p>\n\n\n\n

The Sanger sequencing method is now fully automated. Automation makes it reliable and easy to perform, and it is the 'gold standard' of modern sequencing. With automation and increased sequencing capacity, the cost of the method has also decreased.<\/p>\n\n\n\n

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Indications<\/h3>\n\n\n\n
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